Metabolism of Cholesta-4,7-dien-3-one and Cholesta-4,6-dien-3-one by Mouse Liver Microsomes.
نویسنده
چکیده
منابع مشابه
Oxysterols from human bile induce apoptosis of canine gallbladder epithelial cells in monolayer culture.
Oxysterols have been detected in various mammalian organs and blood. Biliary epithelium is exposed to high concentrations of cholesterol, and we have identified three keto-oxysterols (cholest-4-en-3-one, cholesta-4,6-dien-3-one, cholesta-3,5-dien-7-one) in human bile and gallstones. Because the effects of oxysterols on biliary physiology are not well defined, we investigated their biological ef...
متن کاملBiosynthesis of cholestanol from intestinal 7 alpha-hydroxy-4-cholesten-3-one.
Using isotope dilution-mass spectrometry, it was shown that human bile contains significant amount of 7 alpha-hydroxy-4-cholesten-3-one, an intermediate in the major pathway for bile acid biosynthesis. In bile from 14 healthy subjects, the concentration was 0.14 +/- 0.01 micrograms/ml (mean +/- S.E.). Four bile samples collected from two patients with cerebrotendinous xanthomatosis contained co...
متن کاملSilver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol.
We report that silver ion HPLC provides remarkable separations of C27 sterols differing only in the number or location of olefinic double bonds. This technique has been extended to LC-MS, analysis of purified components by GC, GC-MS, and 1H NMR, and to its use on a semipreparative scale. The application of this methodology for the demonstration of the catalysis, by rat liver microsomes, of the ...
متن کاملThe administration to rats of cholesta-3:5-dien-7-one.
The fact that cholesta-3:5-dien-7-one occurs in arteriosclerotic aorta and some other tissues, in small amounts (Hardegger, Ruzicka & Tagmann, 1943; Prelog, Ruzicka & Stein, 1943), and that it is not necessarily an artifact (Kantiengar & Morton, 1955a) makes it desirable to study the effect of administering it to animals. In the present work it has been given to rats orally at the rate of 25 mg...
متن کاملThe Fate of the 6a-Hydrogen of 5&holest-7-en-3@ol in the Conversion to 7-Dehydrocholesterol by Rat Liver Microsomes*
2-14C-Mevalonic acid has been incubated with a rat liver homogenate in the presence of the inhibitor frans-1,4bis(2 chlorobenzylaminoethyl) cyclohexanedihydrochloride yielding 1,7,15,22 ,26-14CS-cholesta-5,7-dien3@-01, which was reduced with lithium and ammonia to 14Cs-5ar-cholest-7en-30-01 (A’-cholestanol). Separation from endogenous cholesterol was achieved by oxidation with chromic acid, and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of lipid research
دوره 4 شماره
صفحات -
تاریخ انتشار 1963